Summary
Overview
Work History
Education
Skills
Websites
Others
Courses And Diplomats
Advisory Services
Publications
References
Certification
Interests
Timeline
Generic
Pablo Alviz Gazitúa

Pablo Alviz Gazitúa

Biochemist, M. Sc., Ph. D.
Osorno

Summary

Professional with experience in environmental and clinical samples using molecular biology, microbiology, and analytical chemistry techniques. Experienced in laboratory setup, team leadership, and personnel training.

Overview

16
16
years of professional experience
37
37
years of post-secondary education
1
1
Certification
1
1
Language

Work History

Postdoctoral Researcher

Universidad De Los Lagos
12.2023 - Current

Designs and implements a cheap and fast DNA extraction protocol to process 250 samples for metagenomic analysis.
Co-writes a proposal for IDeA R&D Advanced Technologies 2024 Competition .
Designs and coordinate the isolation of a native yeast from Chiloé, also implementing hierarchization of a panel of 325 isolates.


Research assistant

Bacterial Communication Laboratory, Faculty of Sciences, Universidad de Chile
4 2010 - 8 2010
  • Transcript quantification in acidophiles biofilms by RT-qPCR of genes involved in bioleaching

Post Doctoral Researcher

Universidad De Los Lagos, Fondef ID20I10127
11.2020 - 7 2022

Responsible for optimizing the biosynthesis process of bioactive products of bacterial origin.
Conducts molecular biology techniques for the study of Pseudoalteromonas sp.: PCR & genetic modification.
Manages the laboratory and inventory to expedite timelines and organize resources for project execution.
Leads the bidding process to acquire supplies, equipment, and reagents for project execution.
Publishes a review article (Q1) as the first author, connecting sessile metabolism to the production of bioactive compounds in Pseudoalteromonas sp.
Co-author of a selective bacteriostatic product against Vibrio for marine aquaculture, resulting in licensed use.

Post Doctoral Researcher

Universidad de Los Lagos, Fondef ID21I10320
8 2022 - 11.2023

Develop and characterize a collection of 40 isolates of plant growth-promoting microorganisms.
Lead two greenhouse trials from planting to harvest to evaluate the effect of prototype bio-inputs on drought resistance in pasture crops.
Responsible for coordinating and managing supplies and reagents for the laboratory to streamline projects and testing processes. Additionally, conducts negotiations with suppliers and submits requests to achieve results.
Carries out administrative management of the laboratory to adjust timelines and internal procedures for the acquisition and availability of resources.
Awards and executes an internal university project as the principal investigator for the project "Bioprospecting of diazotrophic bacteria adapted to cold climates in soils of the Los Lagos region."
Coordinates and executes the mass cultivation of bacteria for their evaluation as bio-inputs in field trials.

Start up coordinator

Molecular biology laboratory, Atacama Regional Hospital
07.2020 - 10.2020

Leads the process of acquiring equipment, reagents, supplies, and setting up the project, obtaining BSL3 accreditation from the Public Health Institute (ISPCh).
Successfully standardizes the technique for diagnosing SARS-CoV-2 by RT-qPCR in clinical samples.
Directs and trains the team to generate accurate results in a clinical context.
Conducts thorough analyses of samples, providing timely results in the context of the health emergency.

Post doctoral researcher

Molecular Microbiology and Environmental Biotechnology Laboratory, Chemistry Department, Universidad Técnica Federico Santa María
09.2018 - 04.2020

Successfully standardizes protocols for high-performance liquid chromatography coupled with diode-array detection (HPLC-DAD) for the quantification of hydrocarbons.
Designs and conducts bacterial degradation assays of aromatic hydrocarbons in the presence of heavy metals.
Responsible for organizing and planning the maintenance of equipment to extend their useful life.
Remotely coordinates qPCR and scanning electron microscopy (SEM) assays.
Presents a poster at the international congress ISME_Lat2019.
Publishes three scientific articles (Q2, Q2 & Q1), two as the first author, with one being recognized as the most read article in the journal internationally in 2022.

Ph.D. researcher

Bacterial Communication Laboratory, Faculty of Sciences, Universidad de Chile & Biofilms Research Group, University of Calgary, Calgary
04.2013 - 07.2018

Develops a protocol to compare heavy metal resistance in biofilms versus planktonic cells in a panel of mutant strains.
Conducts bioinformatic analyses on the proteome of Cupriavidus metallidurans CH34.
Manages the acquisition of genetic modification tools with international collaborators.
Genetically modifies the species of interest and evaluates phenotypes associated with biofilm formation.
Conducts bright-field and fluorescence microscopy.
Responsible for strain collection.

Master Researcher

Soil Microbial Ecology Group, Basque Institute of Agricultural Research and Development, (NEIKER)
09.2011 - 09.2012

Carries out physiological and enzymatic protocols for soil health evaluation.
Standardizes the qPCR technique to quantify chitinase genes in soil.
Determines the diversity of bacterial communities using the DGGE technique (Denaturing Gradient Gel Electrophoresis System).

Research assistant

Bioengineering Laboratory, Science and Engineering Faculty, Universidad Adolfo Ibañez
10.2010 - 03.2011
  • Carrying out of a clones library from a copper resistance gene (copA) in sandy sediments.

Professional tittle candidate

Molecular genetics Sub-department, Instituto de Salud Pública de Chile
07.2008 - 06.2010
  • Setting up a Mycobacterial DNA extraction and PCR protocol from 15 loci
  • Bioinformatic analysis of minisatellites markers among 150 isolates

Education

Ph.D. - Microbiology

Universidad De Chile
Santiago, Chile
05.2001 - 05.2018

Master of Science - Environmental Agro-Biology

Universidad Del País Vasco
Bilbao, Spain
05.2001 - 05.2012

Bachelor of Science - Biochemistry

Pontificia Universidad Católica De Valparaíso
05.2001 - 05.2010

Skills

    Project Development
    R&D
    Technology Transfer
    Analytical Chemistry
    Molecular Biology
    Microbiology
    Laboratory Setup and Management

Others

  • Driver license
  • Brass musician

Courses And Diplomats

  • Biobusiness Program, Universidad de Los Andes (Chile) & University of San Diego California (USA), 01/04-to date
  • Intellectual & Industrial Property, Universidad de Los Lagos, 10/2023,12/2023
  • Descriptive statitics by R programming, Udemy e-learning, 04/2023, 12/2023
  • Basis of ISO17025:2018 norm, 04/2023, 04/2023
  • Fundamentals of Project Planning and Management, Coursera e-Learning & University of Virginia, Darden School of Business, 10/2021, 12/2021
  • Technology transfer: From research to the market., Coursera e-Learning & Pontificia Universidad Católica de Chile., 09/2019, 12/2019
  • Introduction to patents., Instituto Nacional de la Propiedad Intelectual (INAPI), Ministerio de Economía y Turismo, Chile., 01/2020, 03/2020
  • Native Microbial Biodiversity: Molecular biology tools to characterize plant interacting microoganisms., Centro para el Estudio de Zonas Áridas (CEAZA), Universidad de la Serena., 11/2015
  • Formation of experts in biotechnology patents, Instituto Nacional de la Propiedad Intelectual (INAPI), Ministerio de Economía y Turismo, Chile., 06/2015, 08/2015

Advisory Services

  • Experterra Spa, 07/2023, Present, Prototyping and technical advisoring for in situ NIRS calibration
  • AgroVeca Spa, 02/2022, 04/2022, Technical requirements for the implementation of a bio-based product manufacture facility
  • Agri Marin Terra S.A, 01/2019, 03/2019, Standardization of genotyping protocols for agricultural bioproducts
  • BioPacific S.A., 01/2020, 03/2020, Biosurfactant quantification in agricultural bioproducts

Publications

  • Dynamics and drivers of mycorrhizal fungi after glacier retreat. Carteron A., Cantera I., Guerrieri A., Marta S., Bonin A., Ambrosini R., Anthelme F., Azzoni R.S., Almond P., Alviz Gazitúa P. et al. New Phytologist, 242(4), 1739-1752.
  • Geographical variability of bacterial communities of cryoconite holes of Andean glaciers, F. Pittino, R. Ambrosini, M. Seeger, R. S. Azzoni, G. Diolaiuti, P. Alviz-Gazitua, A. Franzetti, Sci Rep, 10.1038/s41598-022-24373-5
  • The importance of species addition versus replacement varies over succession in plant communities after glacial retreat, I.Cantera, A. Carteron, A. Guerrieri, S. Marta, A. Bonin, R. Ambrosini, F. Anthelme, R. S. Azzoni, P. Almond, P. Alviz Gazitúa et al., Nature Plants, ACCEPTED
  • Local climate modulates the development of soil nematode communities after glacier retreat, A.Guerrieri,I. Cantera, S.Marta, A. Bonin, A. Carteron,R. Ambrosini,M. Caccianiga,F.Anthelme, R. Azzoni, P. Almond., P. Alviz Gazitúa, et al., Global Change Biology, ACCEPTED
  • Enhanced exoelectrogenic activity of Cupriavidus metallidurans in bioelectrochemical systems through the expression of a constitutively active diguanylate cyclase, AlvizGazitua P., Espinoza-Tofalos A., Formicola F., Guiliani N., Turner, R. J., Franzetti, A., Seeger, M., Environments, 10.3390/environments9070080
  • Molecular relationships in biofilm formation and the biosynthesis of exoproducts in Pseudoalteromonas spp, Alviz-Gazitua P., González A., Lee M. R., Aranda C. P., Mar. Biotechnology, 10.1007/s10126-022-10097-0
  • Cupriavidus metallidurans CH34 possesses aromatic catabolic versatility and degrades benzene in the presence of mercury and cadmium, Alviz-Gazitua P., Durán R.E, Millacura F.A, Cárdenas F., Rojas L.A., Seeger M., Microorganisms, 10.3390/microorganisms10020484
  • The Retreat of Mountain Glaciers since the Little Ice Age: A Spatially Explicit Database, Marta S., Azzoni R. S., Fugazza D., Tielidze L., Chand P., Sieron K., Almond P., Ambrosini R., Anthelme F., Alviz-Gazitua, P., et al., Data, 10.3390/data6100107
  • Bio-electrochemical remediation of petroleum hydrocarbons, Espinoza-Tofalos Anna, Alviz-Gazitua Pablo, Franzetti Andrea, Seeger Michael, Bioelectrochemical Systems, Prasun Kumar, Elsevier, Singapore, Republic of Singapore, 10.1007/978-981-15-6868-8
  • The response of Cupriavidus metallidurans CH34 to cadmium involves inhibition of the initiation of biofilm formation, decrease in intracellular c-di-GMP levels and a novel metal regulated phosphodiesterase, Alviz Gazitúa, P., Fuentes-Alburquerque S., Rojas L.A, Turner, R.J., Guiliani N., and Seeger, M., Front Microbiol, 10.3389/fmicb.2019.01499
  • Detection of genes associated with drug resistance in Mycobacterium tuberculosis strains isolated in Chile, P. Araya, M. Velasco, J. Tognarelli, F. Arias, T. Leiva, A. Sccapatticio, P. Alviz, J. Fernández, Rev Med Chile, 139, 467-473, 10.4067/S0034-98872011000400008

References

  • Dr. Michael Seeger, Departament of Chemistry / Universidad Técnica Federico Santa María, michael.seeger@usm.cl, +56 322654223
  • Dr. Nicolás Guiliani, Science Faculty, Department of Biology / Universidad de Chile, nguilian@uchile.cl, +56 229787241
  • Dr. Raymond Turner, Biological Sciences Department/ University of Calgary, turnerr@ucalgary.ca

Certification

[Area of expertise] License - [Timeframe]

Interests

Swiming

Music

Timeline

Postdoctoral Researcher

Universidad De Los Lagos
12.2023 - Current

Post Doctoral Researcher

Universidad De Los Lagos, Fondef ID20I10127
11.2020 - 7 2022

Start up coordinator

Molecular biology laboratory, Atacama Regional Hospital
07.2020 - 10.2020

Post doctoral researcher

Molecular Microbiology and Environmental Biotechnology Laboratory, Chemistry Department, Universidad Técnica Federico Santa María
09.2018 - 04.2020

Ph.D. researcher

Bacterial Communication Laboratory, Faculty of Sciences, Universidad de Chile & Biofilms Research Group, University of Calgary, Calgary
04.2013 - 07.2018

Master Researcher

Soil Microbial Ecology Group, Basque Institute of Agricultural Research and Development, (NEIKER)
09.2011 - 09.2012

Research assistant

Bioengineering Laboratory, Science and Engineering Faculty, Universidad Adolfo Ibañez
10.2010 - 03.2011

Professional tittle candidate

Molecular genetics Sub-department, Instituto de Salud Pública de Chile
07.2008 - 06.2010

Ph.D. - Microbiology

Universidad De Chile
05.2001 - 05.2018

Master of Science - Environmental Agro-Biology

Universidad Del País Vasco
05.2001 - 05.2012

Bachelor of Science - Biochemistry

Pontificia Universidad Católica De Valparaíso
05.2001 - 05.2010

Research assistant

Bacterial Communication Laboratory, Faculty of Sciences, Universidad de Chile
4 2010 - 8 2010

Post Doctoral Researcher

Universidad de Los Lagos, Fondef ID21I10320
8 2022 - 11.2023
Pablo Alviz GazitúaBiochemist, M. Sc., Ph. D.